Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-097-1828   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Human Bone Morphogenetic Protein 4

Recombinant Human Bone Morphogenetic Protein 4

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Cat. No.:
BMP4-23H
Product Overview:
Recombinant human BMP4 is a protein expressed and purified fromhuman 293 cellsas a glycosylated homodimer with an apparent molecular mass of 34-38 kDa.
Description:
Bone Morphogenetic Protein 4 (BMP-4) is a polypeptide belonging to the TGF-β protein superfamily. BMP-4 is involved in bone and cartilage development; more specifically, in tooth and limb development fracture repair1. In human embryonic development, BMP-4 is a critical signaling molecule required for the early differentiation of the embryo and establishment of a dorsal-ventral axis 2,3. BMP-4 plays an important role in differentiation of overlying ectodermal tissue. Inhibition of the BMP-4 signal causes the ectoderm to differentiate into the neural plate. In cultured stem cells, BMP-4 plays a distinct role in mouse and human embryonic stem (ES) cells. BMP-4 supports LIF as a positive factor for mouse ES cell self-renewal4. In contrast, BMP-4 induces extra-embryonic trophoblast differentiation in human ES cells.
Source:
Human 293 cells.
Purity:
Greater than 95 % by SDS-PAGE.
Formulation:
Lyophilized from sterile-filtered 50 mM NaOAc, pH 4.5 and 1 M NaCl containing no additives.
Amino Acid Sequence:
SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFY CHGDCPFPLAD HLNST NHA IVQTLVN SVN SSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR.
Endotoxin Level:
< 1 EU/μg of BMP-4 as determined by the LAL method.
Biologic Activity:
ED50 determined by its ability to induce alkaline phosphatase production by mouse chondrongenic ATDC-5 cells is 4.2 ng/ml.
Storage And Handling:
Stemfactor™ BMP-4, Human Recombinant, is stable in the lyophilized form for six months at -20°C to -80°C. Centrifuge briefly and reconstitute BMP-4 in 4 mM HCl to prepare a working stock solution of no less than 0.1 mg/ml. Reconstituted BMP-4 should be stored in working aliquots at -20°C for up to six months. Multiple freeze and thaw cycles will result in significant loss of activity.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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