Active Recombinant Human CD40LG, FLAG-tagged

• Cat.No.: CD40LG-193H
• Product Overview: Recombinant Human CD40LG(aa 116-261) fussed with mouse ACRP30headless (aa 18-111) and a FLAG-tag at N-terminal was expressed in CHO cells.

Specification

• Description: It is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells (TFH cells). On TFH cells, CD154 promotes B cell maturation and function by engaging CD40 on the B cell surface and therefore facilitating cell-cell communication. A defect in this gene results in an inability to undergo immunoglobulin class switching and is associated with hyper IgM syndrome. Absence of CD154 also stops the formation of germinal centers and therefore prohibiting antibody affinity maturation, an important process in the adaptive immune system.
• Source: CHO cells
• Species: Human
• Tag: FLAG
• Form: Lyophilized. Contains PBS
• Bio-activity: Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: <1ng/ml).
• Molecular Mass: ~35-40kDa (SDS-PAGE)
• Endotoxin: <0.01EU/μg purified protein (LAL test; Lonza).
• Purity: ≥95% (SDS-PAGE)
• Notes: After reconstitution, prepare aliquots and store at -20 centigrade. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions.
• Stability: Stable for at least 6 months after receipt when stored at -20 centigrade.
• Storage: Short Term Storage +4 centigrade;Long Term Storage -20 centigrade.
• Concentration: 0.1mg/ml after reconstitution.
• Reconstitution: Reconstitute with 100μl sterile water.

Gene Information

• Gene Name: CD40LG CD40 ligand [ Homo sapiens ]
• Official Symbol: CD40LG
• Synonyms: IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L; CD40 ligand; CD40 antigen ligand; CD40-L; T-B cell-activating molecule; T-cell antigen Gp39; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5
• Gene ID: 959
• mRNA Refseq: NM_000074
• Protein Refseq: NP_000065
• MIM: 300386
• UniProt ID: P29965
• Chromosome Location: Xq26
• Pathway: Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Asthma, conserved biosystem
• Function: CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding