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Recombinant Human Polymerase (DNA Directed), Beta

Cat.No. : POLB-620H
Product Overview : Recombinant Human Polymerase (DNA Directed), Beta is produced inE. coli.MW=39kDa.
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Cat. No. : POLB-620H
Description : During base excision repair (BER) DNA polymerase β (Pol β) is recruited by APE1. Pol β has two distinct domains, the larger is the polymerase domain itself, whereas a small basic N-terminal domain contains an AP lyase activity that excises the abasic sugar-phosphate residue at the strand break. Pol β also interacts with XRCC1, DNA ligase III and poly(ADP-ribose) polymerase in the repair of DNA single strand breaks. Pol β plays a role in both BER pathways, "single-nucleotide" and "long patch". It is the most inaccurate of the six DNA polymerases found in mammalian cells. Overexpression of Pol β, as found in some human tumors, could confer an increase in spontaneous mutagenesis. It has been further suggested that Pol β does not excise 5'-oxidized AP sites induced by reactive oxygen species (ROS).
Source : E. coli.
Purity : ≥98% (SDS-PAGE).
Concentration : 0.35mg/ml.
Formulation : Liquid. In 20mM TRIS, pH 7.5, containing 20% (v/v) glycerol, 1mM DTT and 100mM sodium chloride.
Stability : Stable for more then 1 year when stored at -20°C or -80°C. Avoid more than 5 freeze/thaw cycles.
Gene Name : POLB polymerase (DNA directed), beta [ Homo sapiens ]
Synonyms : POLB; polymerase (DNA directed), beta; MGC125976; DNA polymerase beta; DNA pol beta; DNA polymerase beta subunit; EC 2.7.7.7
Gene ID : 5423
mRNA Refseq : NM_002690
Protein Refseq : NP_002681
MIM : 174760
UniProt ID : P06746
Chromosome Location : 8p11.2
Pathway : Base excision repair; DNA Repair
Function : DNA-directed DNA polymerase activity; damaged DNA binding; enzyme binding; lyase activity; metal ion binding; microtubule binding; nucleotidyltransferase activity; protein binding; transferase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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09/07/2023

    Efficacy: The enzyme is still active and I am currently using it in some DNA repair assays. It works very well for single nucleotide insertions in gapped DNA which is mostly what I have been using it for recently.
    Quality: Very happy with the quality and longevity. I think the enzyme is ~1yr old with frequent usage and it is still working fine.
    Overall Experience: Very happy. When we need more polB/hUNG, and potentially others, we will purchase from you again.

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