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Recombinant Human Peroxiredoxin 4, His-tagged

Cat.No. : PRDX4-2048H
Product Overview : Recombinant human PRDX4, fused to His-tag at N-terminus, was expressed inE.coliand purified by conventional chromatography techniques, 28.8kDa (255aa).
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Cat. No. : PRDX4-2048H
Description : PRDX4 is an antioxidant enzyme and belongs to the peroxiredoxin (Prdx, Prx, or TrxPx) family. This protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein regulates the of the transcription factor NF-kappaB in the cytosol by a modulation of I-kappa-B-alpha phosphorylation. The ubiquitously expressed peroxiredoxins have also been shown to play a role in apoptosis and cell differentiation.
Source : E.coli.
Form : Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol.
Purity : > 90% by SDS - PAGE.
Concentration : 1 mg/ml (determined by Bradford assay).
Sequences Of Amino Acids : MGSSHHHHHH SSGLVPRGSH MWETEERPRT REEECHFYAG GQVYPGEASR VSVADHSLHL SKAKISKPAP YWEGTAVIDG EFKELKLTDYRGKYLVFFFY PLDFTFVCPT EIIAFGDRLE EFRSINTEVV ACSVDSQFTH LAWINTPRRQ GGLGPIRIPL LSDLTHQISK DYGVYLEDSGHTLRGLFIID DKGILRQITL NDL PVGRSVD ETLRLVQAFQ YTDKHGEVCP AGWKPGSETI IPDPAGKLKY FDKLN.
Biological activity : Specific activity: approximately 230-310 pmole/min/µg. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX4 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25°C for 1 minute.
Activity Assay : 1.Prepare a 50ul reaction mix into a suitable container: the final concentrations are 1mM DTT, 0.03X PBS, 0.5% glycerol.2.Add 5 ul of recombinant PRDX4 solution with various concentrations (0.25ug, 0.5ug) in 45 ul reaction buffer.3.Incubate at 25°C for 2 minutes.4.Add 5ul of 5 mM H2O2 as a substrate and incubate the mixture for 20 min.5.Add 20ul of 26 % trichloroacetic acid (TCA) to stop the reaction. 6.Add 30ul of Formation solution (10mM Ferrous ammonium sulfate (Fe(II)(NH4)2(SO4)2), 2.5M KSCN)7.Record the increase in A475nm.
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : PRDX4 peroxiredoxin 4 [ Homo sapiens ]
Synonyms : peroxiredoxin 4; PRX-4; AOE37-2; PRDX4; peroxiredoxin-4; prx-IV; peroxiredoxin IV; antioxidant enzyme AOE372; thioredoxin peroxidase AO372; thioredoxin peroxidase (antioxidant enzyme); thioredoxin-dependent peroxide reductase A0372; Antioxidant enzyme AOE372; Thioredoxin peroxidase AO372; Prx-IV; Thioredoxin-dependent peroxide reductase A0372; EC 1.11.1.15
Gene ID : 10549
mRNA Refseq : NM_006406
Protein Refseq : NP_006397
MIM : 606506
UniProt ID : Q13162
Chromosome Location : Xp22.11
Function : oxidoreductase activity; thioredoxin peroxidase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What is the primary biological role of PRDX4 in cells? 05/09/2022

PRDX4 acts as a peroxidase, reducing peroxides to protect cells from oxidative stress.

What role does PRDX4 play in protein folding and quality control? 08/15/2020

It assists in protein folding and prevents the aggregation of misfolded proteins.

In which cellular compartments is PRDX4 primarily located? 07/14/2020

PRDX4 is primarily located in the endoplasmic reticulum and secreted extracellularly.

Are there potential therapeutic applications targeting PRDX4? 06/15/2020

Therapeutic applications may involve modulating PRDX4 activity to mitigate oxidative stress-related diseases.

How does PRDX4 contribute to the cellular antioxidant defense system? 10/18/2019

It reduces hydrogen peroxide and lipid peroxides, contributing to the antioxidant defense.

How is the activity of PRDX4 regulated within cells? 01/01/2019

Cellular redox status and covalent modifications regulate PRDX4 activity.

What happens when there is dysregulation or deficiency of PRDX4? 10/13/2018

PRDX4 deficiency can lead to increased oxidative stress and damage, affecting cellular functions.

Customer Reviews (3)

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Reviews
12/07/2021

    Delivery on time, perfect.

    01/19/2021

      Very effective protein product.

      10/24/2020

        High-quality, great for research.

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