Monoclonal antibodies are produced by immunizing mouse with synthesized polypeptide 6 x His, coupled with KLH. This antibody can recognize protein with His-tag in C-terminal.Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibody have been widely used in study of protein expression in various systems. Our anti-His monoclonal antibody provides a simple solution to detect the expression of protein with a His tag.
Western Blot, Immunoprecipitation.
1×PBS with 50% glycerol, protein concentration: 0.5 mg/ml.
Recommended Antibody Dilution:
Western Blot: 0.5μg/ml.
Antibody is recommended being stored at -20℃. Avoid repeated freeze-thaw cycle.