Recombinant Human GPC3 (P51654-1) (Met 1-His 559), fused with a C-terminal polyhistidine tag, was produced in Human Cell.
Lyophilized from sterile PBS, pH 7.4
The secreted recombinant human GPC3 comprises 546 amino acids and has a predicted molecular mass of 62 kDa. GPC3 is composed of proteolytic cleaved two subunits linked by disulfide bonds, that are produced by proprotein convertases. As a result of glycosylation, apparent molecular mass of rhGPC3 is approximately 40 and 50-60 kDa in SDS-PAGE under reducing conditions, corresponding to the N-terminal and C-terminal fragment respectively.
< 1.0 eu per μg of the protein as determined by the LAL method.
Samples are stable for up to twelve months from date of receipt at -70oC.
Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4oC before opening to recover the entire contents.