Active Recombinant Human INHBB protein(Met1-Ala407), His-tagged

• Cat.No.: INHBB-2261H
• Product Overview: Recombinant Human INHBB (NP_002184.2) (Met 1-Ala 407) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Ala407
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human INHBB-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse FLRG-His with a linear range of 0.031-0.125 μg/ml.
• Molecular Mass: The secreted pro form of human INHBB comprises 390 amino acids with a predicted molecular mass of 43.7 kDa. As a result of glycosylation, the apparent molecular mass of rhINHBB is approximately 17, 42 and 55 kDa in SDS-PAGE under reducing conditions, corresponding to the mature form, pro peptide and pro form respectively.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: INHBB inhibin, beta B [ Homo sapiens ]
• Official Symbol: INHBB
• Synonyms: INHBB; inhibin, beta B; inhibin, beta B (activin AB beta polypeptide); inhibin beta B chain; Inhibin, beta-2; activin beta-B chain; activin AB beta polypeptide; MGC157939
• Gene ID: 3625
• mRNA Refseq: NM_002193
• Protein Refseq: NP_002184
• MIM: 147390
• UniProt ID: P09529