Recombinant Human SPP1, His tagged

• Cat.No.: SPP1-664H
• Product Overview: Recombinant Human SPP1 pro form (NP_001035147.1) (Met 1-Asn 314), fused with a polyhistidine tag at the C-terminus, was produced in Human Cell.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human SPP1 consists of 309 amino acids after removal of the signal peptide and has a calculated molecular mass of 35 kDa. The apparent molecular mass of rh SPP1 is approximately 60-65 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Protein Length: Met 1-Asn 314
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: SPP1 secreted phosphoprotein 1 [ Homo sapiens ]
• Official Symbol: SPP1
• Synonyms: SPP1; secreted phosphoprotein 1; BNSP, bone sialoprotein I , OPN, osteopontin; osteopontin; BSPI; early T lymphocyte activation 1; ETA 1; uropontin; nephropontin; osteopontin-C; osteopontin-D; SPP1/CALPHA1 fusion; bone sialoprotein 1; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); OPN; BNSP; ETA-1; MGC110940
• Gene ID: 6696
• mRNA Refseq: NM_000582
• Protein Refseq: NP_000573
• MIM: 166490
• UniProt ID: P10451