| Source : |
HEK293 |
| Species : |
Mouse |
| Tag : |
C-His |
| Protein length : |
Met 1-Pro 297 |
| Form : |
Lyophilized from sterile PBS, pH 7.4. |
| Bio-activity : |
1. Immobilized Rituximab (IgG1) at 1 μg/mL can bind Mouse FcγRI / CD64 recombinant protein, the EC50 is 16-35 ng/mL. 2. Immobilized Anti-CD3 antibody, mouse IgG2a at 2 μg/mL (100 μL/well) can bind Recombinant Mouse CD64 / FCGR1 Protein (His Tag), the EC50 is 0.8-2.4 ng/mL. 3. Loaded Mouse FcγRI / CD64 recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.12 μM as determined in a BLI assay (Sartorius Octet Red384). 4. Loaded Mouse FcγRI / CD64 recombinant protein on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 0.28 μM as determined in a BLI assay (Sartorius Octet Red384). 5. Captured Mouse FcγRI / CD64 recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.12 μM as determined in an SPR assay (Biacore T200). 6. Captured Mouse FcγRI / CD64 recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.69 μM as determined in an SPR assay (Biacore T200) (Routinely tested). 7. The purity of Mouse CD64 / FCGR1 Protein (His Tag) is more than 95% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS. 8. Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) on ProA Biosensor, can bind Recombinant Mouse CD64 Protein, His Tag with an affinity constant of 3.46nM as determined in BLI assay (Sartorius Octet RED384). 9. Loaded Recombinant Mouse CD16/FCGR3 Protein, His Tag on His1K Biosensor, can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) with an affinity constant of 18.5nM as determined in BLI assay (Sartorius Octet RED384). |
| Molecular Mass : |
The recombinant mouse FCGR1 consists of 284 amino acids after removal of the signal peptide and has a predicted molecular mass of 32 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FCGR1 is approximately 45-50 kDa due to glycosylation. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : |
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
