Recombinant Human METAP2(aa 1-478) fused with hemagglutinin (HA) tag (YPYDVPDYA) at N-terminal was expressed in Insect cells.
MetAP2 is a metalloenzyme that catalyzes removal of the N-terminal methionine from proteins, with the physiologically relevant metal thought to be either cobalt or manganese. Since it was identified as the target of the anti-angiogenic natural product fumagilin (Prod. No. BML-CT100), it has been studied for its involvement in cancer, angiogenesis, inflammation, and rheumatoid arthritis. Because of differing substrate specificities and kinetics it is not desirable to substitute bacterial methionine aminopeptidase for mammalian MetAP2.
Liquid. In 20mM HEPES, pH 7.3, containing 150mM sodium chloride, 10% glycerol, 0.02% sodium azide, and 0.1mM CoCl2.
Stable for at least 6 months after receipt when stored at -80 centigrade.
Long Term Storage: -80 centigrade; Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80 centigrade.