Recombinant Human FASLG, FLAG-tagged

• Cat.No.: FASLG-2424H
• Product Overview: Recombinant extracellular domain of human FasL (aa 103-281) produced inHEK 293 cellsis fused at the N-terminus to a linker peptide (26 aa) and a FLAG-tag. Glycosylation of rhsSuperFasLigandTM is similar to natural human FasL. 32kDa (nonglycosylated), 35kDa (glycosylated).

Specification

• Cat. No.: FASLG-2424H
• Description: Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. The Fas molecule is comprised of 319 amino acids of which 145 amino acids constitute the cytoplasmic region, 17 amino acids the transmembrane domain and 157 amino acids the extracellular region. The extracellular portion of Fas is called sFasR. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD and processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL.
• Source/Host: HEK 293 cells.
• Concentration: 0.1mg/ml after reconstitution.
• Purity: ≥95% (SDS-PAGE).
• Endotoxin Content: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
• Reconstitution: Reconstitute with 50µl sterile water. Further dilutions should be made with cell culture medium containing 5% fetal calf serum.
• Specificity: Binds to human, mouse and rat Fas (CD95; APO-1).
• Formulation: Lyophilized. Contains PBS.
• Biological Activity: Kills Fas-sensitive cells at concentrations of >1ng/ml. Note: Does not require enhancer.
• Specific Activity: ED50: 1ng/ml (A20 cells).
• Long Term Storage: -20°C.
• Use/Stability: Stable for at least 6 months after receipt when stored at -20°C.
• Handling: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Gene Information

• Gene Name: FASLG Fas ligand (TNF superfamily, member 6) [ Homo sapiens ]
• Synonyms: FASLG; Fas ligand (TNF superfamily, member 6); FASL; CD178; CD95L; TNFSF6; APT1LG1; fas ligand; CD95 ligand; apoptosis (APO-1) antigen ligand 1; tumor necrosis factor (ligand) superfamily, member 6; CD95L protein; APTL;CD_antigen; Tumor necrosis factor ligand superfamily member 6, membrane form; Tumor necrosis factor ligand superfamily member 6, soluble form
• Gene ID: 356
• mRNA Refseq: NM_000639
• Protein Refseq: NP_000630
• MIM: 134638
• UniProt ID: P48023
• Chromosome Location: 1q23
• Pathway: Allograft rejection; Apoptosis; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; MAPK signaling pathway; Natural killer cell mediated cytotoxicity; Neurotrophin signaling pathway; Pathways in cancer; Type I diabetes mellitus
• Function: cytokine activity; tumor necrosis factor receptor binding