Recombinant Rat Caspase 3, His-tagged

• Cat.No.: Casp3-748R
• Product Overview: Recombinant rat Casp3 was fused at the N- and C- terminus to a His•Tag sequence and expressed inE. colias a single polypeptide. Subsequently undergoes spontaneous yield the active enzyme. MW = 11000 and 17000.

Specification

• Cat. No.: Casp3-748R
• Description: The CASP3 protein is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7, and 9; and the protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer"s disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
• Source: E.coli.
• Purity: ≥80% by SDS-PAGE.
• Form: Lyophilized.
• Specific Activity: ≥9,000 units/mg protein.
• Unit definition: One unit is defined as the amount of enzyme that will cleave 1 nmol of the substrate DEVD-pNA per h at 37°C, pH 7.2.
• Solubility: Reconstitute in 100 µl PBS (1 unit/µl).
• Toxicity: Standard Handling (MSDS available upon request).
• Recommended Reaction Conditions: 50 mM HEPES, pH 7.2, 50 mM NaCl, 10 mM EDTA, 10 mM DTT, 0.1% CHAPS, and 5% glycerol; 37°C.
• Storage: ≤ -70°C. Following reconstitution aliquot and freeze(-70°C). Stock solutions are stable for up to 6 months at -70°C. Avoid freeze/thaw cycles of solutions.

Gene Information

• Gene Name: Casp3 caspase 3 [ Rattus norvegicus ]
• Synonyms: Casp3; caspase 3; Lice; MGC93645; caspase-3; IRP; SCA-1; CASP-3; CPP-32; apopain; CPP32beta; protein Yama; yama protein; cysteine protease CPP32; SREBP cleavage activity 1; Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease); Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease); EC 3.4.22.56
• Gene ID: 25402
• mRNA Refseq: NM_012922
• Protein Refseq: NP_037054
• UniProt ID: P55213
• Chromosome Location: 16q11
• Pathway: Alzheimer"s disease; Amyotrophic lateral sclerosis (ALS); Apoptosis; Colorectal cancer; Huntington"s disease; MAPK signaling pathway; Natural killer cell mediated cytotoxicity; Parkinson"s disease; Pathways in cancer; Viral myocarditis; p53 signaling pathway
• Function: cyclin-dependent protein kinase inhibitor activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; peptidase activity; phospholipase A2 activator activity; protein binding