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Home > Products > Recombinant Proteins > Recombinant Mouse Cathepsin E, His-tagged

Recombinant Mouse Cathepsin E, His-tagged

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Human(7)
Mouse(7)
Rat(4)
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GST(1)
His(8)
Cat. No.:
Ctse-749M
Product Overview:
Recombinant mouse cathepsin E was fused at the C-terminus to a His•Tag sequence and expressedNSO cells. MW = 44 kDa.
Description:
Cathepsin E is an intracellular aspartic protease of the pepsin family that has been reported to be involved in the cleavage of the Swedish mutant of amyloid precursor protein (APP). Due to glycosylation the apparent molecular weight of the dimer is ~100 kDa under non-reducing conditions. Requires activation prior to use.
Source:
NSO cells.
Purity:
≥95 by SDS-PAGE.
Form:
Liquid. In 75 mM NaCl, 12.5 mM MES, 50% glycerol, pH 6.5, sterile-filtered. Please refer to vial label for lot-specific concentration.
Recommended Reaction Conditions:
Activation Protocol: Pre-incubate Cathepsin E for 15 min. at room temperature, at a concentration of 1 µg/ml in 50 mM sodium acetate buffer, pH 3.5, containing 100 mM NaCl. Activity: Optimal activity is achieved in 50 mM sodium acetate buffer, pH 3.5, containing 100 mM NaCl, room temperature.
Endotoxins:
≤1.0 EU/µg enzyme.
Specific Activity:
≥8 units/μg.
Unit Definition:
One unit is defined as the amount of enzyme that will cleave 1 nmol MCA-PLGL(3-DPA-diaminopropionyl)-AR-NH2 at 25°C, pH 3.5.
Toxicity:
Standard Handling (MSDS available upon request).
Storage:
≤ -70℃. Avoid freeze/thaw. Following initial thaw, aliquot and freeze (-70℃).


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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