Recombinant Human Histone Deacetylase 8, His-tagged
Cat.No. : | HDAC8-759H |
Product Overview : | Recombinant Human histone deacetylase 8 (HDAC8) was fused to at the C-terminus to a His•Tag sequence at and expressed inS. frugiperdainsect cells using a baculovirus expression system. MW = 46400 Da. |
- Specification
- Gene Information
- Related Products
Cat. No. : | HDAC8-759H |
Description : | HDAC8 phosphorylated in vivo by PKA results in decreased activity, which leads to the hyperacetylation of histones H3 and H4. HDAC8 may play a role in one of the most frequent types of acute myeloid leukemia (AML). The active deacetylase is useful in the study of HDAC8 regulation and for inhibitor screening. |
Source : | S. frugiperda. |
Purity : | ≥90% by SDS-PAGE. |
Form : | Liquid. In 138 mM NaCl, 25 mM Tris-HCl, 10% glycerol, 0.05% TWEEN-20 detergent, pH 8.0. Please refer to vial label for lot-specific concentration. |
Specific Activity : | ≥10 units/µg protein. |
Unit Definition : | One unit is defined as the the amount of enzyme required to deacetylate 1 picomol fluorescent peptide substrate [Arg-His-Lys(Ac)-Lys(Ac)] per min at 30°C. |
Recommended Reaction Conditions : | 137 mM NaCl, 25 mM Tris-HCl, 2.7 mM KCl, 1 mM MgCl2, pH 8.0, 100 µM fluorescent substrate [Arg-His-Lys(Ac)-Lys(Ac)], 100 µg/ml BSA, 15 ng/ml HDAC8; incubate at 30°C for 60 min. |
Toxicity : | Standard Handling (MSDS available upon request). |
Storage : | ≤ -70℃. Avoid freeze/thaw. Following reconstitution, aliquot and freeze (-70°C). |
Gene Name : | HDAC8 histone deacetylase 8 [ Homo sapiens ] |
Synonyms : | HDAC8; histone deacetylase-like 1; RPD3; HDACL1; histone deacetylase 8; EC 3.5.1.98; HD8 |
Gene ID : | 55869 |
mRNA Refseq : | NM_018486 |
Protein Refseq : | NP_060956 |
MIM : | 300269 |
UniProt ID : | Q9BY41 |
Chromosome Location : | Xq13 |
Pathway : | Gene Expression |
Function : | histone deacetylase activity; hydrolase activity; transcription factor binding |
Products Types
◆ Recombinant Protein | ||
HDAC8-26H | Recombinant Human HDAC8 Protein, His-tagged | +Inquiry |
HDAC8-2473R | Recombinant Rat HDAC8 Protein, His (Fc)-Avi-tagged | +Inquiry |
HDAC8-314H | Active Recombinant Human HDAC8 Protein | +Inquiry |
HDAC8-4650H | Recombinant Human HDAC8 Protein, GST-tagged | +Inquiry |
HDAC8-125H | Recombinant Human HDAC8 Protein, His-tagged | +Inquiry |
◆ Lysates | ||
HDAC8-537MCL | Recombinant Mouse HDAC8 cell lysate | +Inquiry |
HDAC8-691HCL | Recombinant Human HDAC8 cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-1744 | HDAC8 Fluorogenic Assay Kit | +Inquiry |
Kit-2253 | HDAC8 Activity Fluorometric Assay Kit | +Inquiry |
Kit-1961 | HDAC8 Inhibitor Screening Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
0
Inquiry Basket