Recombinant Human CHEK2, His-tagged

• Cat.No.: CHEK2-686H
• Product Overview: Recombinant human checkpoint homologue kinase 2 (Chk2) fused to a His Tag sequence at the N-terminus was expressed inS. frugiperdainsect cells. MW = 65430 Da.

Specification

• Cat. No.: CHEK2-686H
• Description: Chk2 is involved in sensing DNA damage in eukaryotic cells. It plays an important role in the control of the cell cycle by blocking replication.
• Source: S. frugiperda.
• Form: Liquid. In 270 mM sucrose, 150 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 0.1 mM EGTA, pH 7.5. Please refer to vial label for lot-specific concentration.
• Specific Activity: ≥ 25,000 units/mg protein.
• Purity: ≥ 95% by SDS PAGE.
• Unit Definition: One unit is defined as the amount of enzyme required to transfer 1 picomol phosphate to the synthetic peptide substrate KKKVSRSGLYRSPSMPENLNRPR per min at 30°C.
• Toxicity: Standard Handling (MSDS available upon request).
• Storage: ≤ -70 °C. Avoid freeze/thaw Following initial thaw, aliquot and freeze (-70°C).

Gene Information

• Gene Name: CHEK2 CHK2 checkpoint homolog (S. pombe) [ Homo sapiens ]
• Synonyms: CHEK2; CHK2 checkpoint homolog (S. pombe); CDS1; CHK2; LFS2; RAD53; HuCds1; PP1425; CHK2 (checkpoint, S.pombe) homolog; Serine/threonine-protein kinase Chk2; EC 2.7.11.1; Cds1
• Gene ID: 11200
• mRNA Refseq: NM_001005735
• Protein Refseq: NP_001005735
• MIM: 604373
• UniProt ID: O96017
• Chromosome Location: 22q12.1
• Pathway: Cell cycle; p53 signaling pathway; Cell Cycle Checkpoints
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity; magnesium ion binding; protein binding