Recombinant Mouse Hmgb1(Met1-Glu215) fused with His tag at N-terminal was expressed in E. coli.
Sterile liquid; 20 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 1 mM DTT. 0.22 um filtered.
The ED50 of this protein, as measured by mouse TNF alpha induction in RAW264.7 cells, is 3-25 ug/mL in the presence of 10 ug/mL Polymyxin B. This corresponds to a specific activity of 3,300-40 Units/mg.
Has a predicted molecular mass of 27.6 kDa.
Less than 0.01 ng/ug cytokine as determined by the LAL assay.
> 98%, as determined by SDS-PAGE
Store at less than or equal to -70 centigrade.