"Hmgb1" Related Products

Recombinant Mouse Hmgb1 protein, His-tagged

Cat.No.: Hmgb1-2628M
Product Overview: Recombinant Mouse Hmgb1(Met1-Glu215) fused with His tag at N-terminal was expressed in E. coli.
Source: E. coli
Species: Mouse
Tag: His
Form: Sterile liquid; 20 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 1 mM DTT. 0.22 um filtered.
Bio-activity: The ED50 of this protein, as measured by mouse TNF alpha induction in RAW264.7 cells, is 3-25 ug/mL in the presence of 10 ug/mL Polymyxin B. This corresponds to a specific activity of 3,300-40 Units/mg.
Molecular Mass: Has a predicted molecular mass of 27.6 kDa.
Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Purity: > 98%, as determined by SDS-PAGE
Storage: Store at less than or equal to -70 centigrade.
Concentration: 0.5 mg/mL
Gene Name: Hmgb1 high mobility group box 1 [ Mus musculus ]
Official Symbol: Hmgb1
Synonyms: HMGB1; high mobility group box 1; high mobility group protein B1; high mobility group protein 1; DEF; p30; Hmg1; HMG-1; SBP-1; amphoterin; MGC103168; MGC103169; MGC117896; MGC117897;
Gene ID: 15289
mRNA Refseq: NM_010439
Protein Refseq: NP_034569
MIM:
UniProt ID: P63158
Chromosome Location: 5 G3; 5 89.18 Cm
Pathway: Activated TLR4 signalling, organism-specific biosystem; Activation of DNA fragmentation factor, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis induced DNA fragmentation, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; Base excision repair, organism-specific biosystem;
Function: 5S rRNA binding; DNA binding; DNA binding, bending; RAGE receptor binding; bent DNA binding; binding; calcium-dependent protein kinase regulator activity; crossed form four-way junction DNA binding; cytokine activity; cytokine activity; double-stranded DNA binding; four-way junction DNA binding; glycolipid binding; heparin binding; open form four-way junction DNA binding; peptide binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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