Active Recombinant Mouse Hmgb1 protein, His-tagged

• Cat.No.: Hmgb1-2628M
• Product Overview: Recombinant Mouse Hmgb1(Met1-Glu215) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Sterile liquid; 20 mM Tris-HCl, pH 8.0, 0.2 M NaCl, 1 mM DTT. 0.22 um filtered.
• Bio-activity: The ED50 of this protein, as measured by mouse TNF alpha induction in RAW264.7 cells, is 3-25 ug/mL in the presence of 10 ug/mL Polymyxin B. This corresponds to a specific activity of 3,300-40 Units/mg.
• Molecular Mass: Has a predicted molecular mass of 27.6 kDa.
• Endotoxin: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
• Purity: > 98%, as determined by SDS-PAGE
• Storage: Store at less than or equal to -70 centigrade.
• Concentration: 0.5 mg/mL
• Publication:
  Functional Role of Soluble Receptor for Advanced Glycation End Products in Stroke (2013)

Gene Information

• Gene Name: Hmgb1 high mobility group box 1 [ Mus musculus ]
• Official Symbol: Hmgb1
• Synonyms: HMGB1; high mobility group box 1; high mobility group protein B1; high mobility group protein 1; DEF; p30; Hmg1; HMG-1; SBP-1; amphoterin; MGC103168; MGC103169; MGC117896; MGC117897
• Gene ID: 15289
• mRNA Refseq: NM_010439
• Protein Refseq: NP_034569
• UniProt ID: P63158
• Chromosome Location: 5 G3; 5 89.18 Cm
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Activation of DNA fragmentation factor, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis induced DNA fragmentation, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; Base excision repair, organism-specific biosystem
• Function: 5S rRNA binding; DNA binding; DNA binding, bending; RAGE receptor binding; bent DNA binding; binding; calcium-dependent protein kinase regulator activity; crossed form four-way junction DNA binding; cytokine activity; cytokine activity; double-stranded DNA binding; four-way junction DNA binding; glycolipid binding; heparin binding; open form four-way junction DNA binding; peptide binding