Active Recombinant Human Nerve Growth Factor (Beta Polypeptide)

• Cat.No.: NGF-476H
• Product Overview: Recombinant Human NGF (Ser 122-Arg 239) was expressed in CHO Cells.

Specification

• Cat. No.: NGF-476H
• Description: As the first one of a series of neurotrophic factors, nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF, was demonstrated to exhibits the growth stimulating activity of NGF. β-NGF is a target-derived secreted homodimeric protein of two 120-amino acid mature polypeptides after proteolytic removal of the signal and pro peptide. NGF acts via at least two receptors on the surface of cells, TrkA and p75 receptors to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation.
• Purity: > 98 % as determined by SDS-PAGE
• Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.4 - 1.0 ng / ml Measured in a cell survival assay using PC-12 rat pheochromocytoma cells. The ED50 for this effect is typically 1.07 ng / mL
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70°C
• Predicted N terminal: Ser 122
• Molecular Mass: The mature recombinant human β-NGF consists of 118 amino acids and has a predicted molecular mass of 13.2 kDa. β-NGF exists as a non-disulfide linked homodimer in solution
• Formulation: Lyophilized from sterile 20mM NaAc, 150mM NaCl , pH 5.5
• Storage: Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: NGF nerve growth factor (beta polypeptide) [ Homo sapiens ]
• Synonyms: Beta-NGF; HSAN5; MGC161426; MGC161428; NGFB; NID67; OTTHUMP00000013653; beta-nerve growth factor; nerve growth factor (beta polypeptide); nerve growth factor, beta polypeptide; nerve growth factor, beta subunit; Beta Polypeptide; NGF
• Gene ID: 4803
• mRNA Refseq: NM_002506
• Protein Refseq: NP_002497
• MIM: 162030
• UniProt ID: P01138
• Chromosome Location: 1p13.1
• Pathway: Apoptosis; MAPK signaling pathway; Signalling by NGF
• Function: growth factor activity; nerve growth factor receptor binding; receptor signaling protein activity