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   Your Position: Home >> Hotstart Taq DNA polymerase >> Molecular Biological Reagents >> Hotstart Taq DNA polymerase

Hotstart Taq DNA polymerase

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Cat. No.

CMC15

Description

HotStart Taq DNA Polymerase is a thermostable, modified form of recombinant Taq DNA Polymerase. It is inactive at temperatures below 75oC, but can be activated by a 4 min 95oC incubation step. The combination of HotStart Taq DNA Polymerase and the optimized PCR buffer minimizes non-specific amplification products and primer dimers allowing highest sensitivity.

Formulation

HotStart Taq DNA polymerase, 10x HotStart reaction buffer(without Mg2+), 25mM MgCl2

Application

Amplification of genomic DNA and cDNA targets up to 3 kb long with high specificity, sensitivity, and yield, Real time PCR, Multiplex PCR, Carry-over prevention, Automated PCR e.g. handling at room temperatures.

Example

Use λ-DNA as template to do PCR. PCR condition:94 5min/30cycles  94 30sec, 58 30sec, 72 45sec/72 5min.

Storage

-20




 

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