Recombinant Human MAPKAPK2

• Cat.No.: MAPKAPK2-670H
• Product Overview: Recombinant human MAPKAPK2 (46-end) was expressed inHEK 293 cells.

Specification

• Cat. No.: MAPKAPK2-670H
• Description: MAPKAPK2 is a serine/threonine protein kinase activated by the p38 MAP kinase in response to various signals including mitogenic and stress stimuli. MAPKAPK2 is distinguished from the RSK family of ribosomal protein S6 kinases (also termed MAPKAPK1), which are activated by the MAP kinases, ERK1, and ERK2 based on its substrate specificity and amino acid sequence. Upon activation, p38 MAP kinase activates the downstream MAPKAPK2 by phosphorylation of the threonine residue in the Pro-Thr motif in response to heat shock or mitogenic stimuli. Activated MAPKAPK2 phosphorylates the small heat shock protein HSP25/HSP27, both in vitro and in vivo, at serine residues. Thus, HSP25/HSP27 seems to be the physiological substrate of MAPKAPK2.
• Source: HEK 293 cells.
• Unit Definition: One unit will incorporate 1.0 nmole of phosphate into HSP 25 per minute at 30 °C at pH 7.4.
• Physical Form: Solution in 50 mM Tris-HCl, pH 7.4, 100 mM NaCl, 0.05 mM EDTA, 2 mM DTT, and 10% glycerol.
• Form: Buffered aqueous glycerol solution.
• Storage: −70°C. Shipped in dry ice.

Gene Information

• Gene Name: MAPKAPK2 mitogen-activated protein kinase-activated protein kinase 2 [ Homo sapiens ]
• Synonyms:
  MAPKAPK2; mitogen-activated protein kinase-activated protein kinase 2; MK2; EC 2.7.11.1; MAPKAP kinase 2; MAPKAPK-2
• Gene ID: 9261
• mRNA Refseq: NM_004759
• Protein Refseq: NP_004750
• MIM: 602006
• UniProt ID: P49137
• Chromosome Location: 1q32
• Pathway: MAPK signaling pathway; Neurotrophin signaling pathway; VEGF signaling pathway; Signalling by NGF
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; signal transducer activity; transferase activity