Recombinant Human GPC3 protein, His-tagged
Recombinant Human GPC3(Met1-His559) fused with His tag at C-terminal was expressed in NS0.
Predicted N Terminal:
No results obtained: Gln25 predicted, Ser359 & Val483
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by its binding ability in a functional ELISA. When Recombinant Human Glypican 3 is immobilized at 0.5 μg/mL (100 µL/well), the concentration of Recombinant Human FGF basic 146 aa that produces 50% of the optimal binding response is approximately 0.6-3 ng/mL.
Predicted Molecular Mass: 61.6 kDa;SDS-PAGE: 60-100 kDa, non-reducing conditions.
<0.10 EU per 1 μg of the protein by the LAL method.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 100 μg/mL in sterile PBS.