Active Recombinant Human ANGPT2 protein(Met1-Phe496), His-tagged

• Cat.No.: ANGPT2-3182H
• Product Overview: Recombinant Human Angiopoietin 2 (NP_001138.1) (Met 1-Phe 496) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: C-His
• Protein length: Met1-Phe496
• Form: Lyophilized from sterile 2mM MOPS, 15mM NaCl, .5% CHAPS, pH 7. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind recombinant human Tie2-His in a functional ELISA. 2. Measured by its ability to bind recombinant human Tie2-Fch in a functional ELISA.
• Molecular Mass: The recombinant human Ang2 consists of 489 amino acids and predicts a molecular mass of 56.4 kDa. As a result of glycosylation, the rhAng2 migrates as approximately 63.5 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ANGPT2 angiopoietin 2 [ Homo sapiens ]
• Official Symbol: ANGPT2
• Synonyms: ANGPT2; angiopoietin 2; angiopoietin-2; Ang2; ANG-2; Tie2-ligand; angiopoietin-2B; angiopoietin-2a; ANG2; AGPT2
• Gene ID: 285
• mRNA Refseq: NM_001118887
• Protein Refseq: NP_001112359
• MIM: 601922
• UniProt ID: O15123