Recombinant Human Adenosylhomocysteinase, His-tagged

• Cat.No.: AHCY-727H
• Product Overview: Recombinant human AHCY protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW =49.8 kDa (452aa).

Specification

• Cat. No.: AHCY-727H
• Description: AHCY, also known as adenosylhomocysteinase, is an enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). It regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MSDKLPYKVA DIGLAAWGRK ALDIAENEMP GLMRMRERYS ASKPLKGARI AGCLHMTVET AVLIETLVTL GAEVQWSSCN IFSTQDHAAA AIAKAGIPVY AWKGETDEEY LWCIEQTLYF KDGPLNMILD DGGDLTNLIH TKYPQLLPGI RGISEETTTG VHNLYKMMAN GILKVPAINV NDSVTKSKFD NLYGCRESLI DGIKRATDVM IAGKVAVVAG YGDVGKGCAQ ALRGFGARVI ITEIDPINAL QAAMEGYEVT TMDEACQEGN IFVTTTGCID IILGRHFEQM KDDAIVCNIG HFDVEIDVKW LNENAVEKVN IKPQVDRYRL KNGRRIILLA EGRLVNLGCA MGHPSFVMSN SFTNQVMAQI ELWTHPDKYP VGVHFLPKKL DEAVAEAHLG KLNVKLTKLT EKQAQYLGMS CDGPFKPDHY RY
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol.
• Concentration: 1.0 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: AHCY adenosylhomocysteinase [ Homo sapiens ]
• Synonyms: AHCY; adenosylhomocysteinase; SAHH; S-adenosylhomocysteine hydrolase; EC 3.3.1.1; AdoHcyase; S-adenosyl-L-homocysteine hydrolase; OTTHUMP00000030684
• Gene ID: 191
• mRNA Refseq: NM_000687
• Protein Refseq: NP_000678
• MIM: 180960
• UniProt ID: P23526
• Chromosome Location: 20cen-q13.1
• Pathway: Methionine metabolism; Selenoamino acid metabolism; Biological oxidations
• Function: adenosylhomocysteinase activity; copper ion binding; hydrolase activity; protein self-association