Recombinant E.coli Periplasmic Chaperone, His-tagged

• Cat.No.: Skp-2573E
• Product Overview: Recombinant E.coli Skp, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 17.9 kDa (162aa).

Specification

• Cat. No.: Skp-2573E
• Description: The Seventeen Kilodalton Protein (Skp) is a trimeric periplasmic chaperone that assists outer membrane proteins in their folding and insertion into membranes. This protein required for the efficient release of ompA from the inner membrane, the maintenance of its solubility in the periplasm, and, in association with lipopolysaccharide (LPS), for the efficient folding and insertion of ompA into the outer membrane.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MADKIAIVNM GSLFQQVAQK TGVSNTLENE FKGRASELQR METDLQAKMK KLQSMKAGSD RTKLEKDVMA QRQTFAQKAQ AFEQDRARRS NEERGKLVTR IQTAVKSVAN SQDIDLVVDA NAVAYNSSDV KDITADVLKQ VK
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol.
• Concentration: 1mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: skp periplasmic chaperone [ Escherichia coli str. K-12 substr. MG1655 ]
• Synonyms: Skp; periplasmic chaperone; ECK0177; hlpA; JW0173; oomph; periplasmic molecular chaperone for outer membrane proteins
• Gene ID: 944861
• Protein Refseq: NP_414720