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Recombinant Human FK506 Binding Protein 14, 22 kDa, His-tagged

Cat.No. : FKBP14-2574H
Product Overview : Recombinant human FKBP14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. MW = 24.2 kDa (213aa).
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  • Gene Information
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Cat. No. : FKBP14-2574H
Description : FKBP14, also known as 22 kDa FK506-binding protein, is an enzyme that accelerates the folding of proteins during protein synthesis. This protein contains two EF-hand domains and one PPIase FKBP-type domain. Truncation of the amino-terminus of FKBP14 greatly reduces peptidyl prolyl cis-trans isomerase activity, therefore suggesting that the PPIase FKBP-type domain must be located at the N-terminus.
Source : E.coli.
Sequences Of Amino Acids : MGSSHHHHHH SSGLVPRGSH MALIPEPEVK IEVLQKPFIC HRKTKGGDLM LVHYEGYLEK DGSLFHSTHK HNNGQPIWFT LGILEALKGW DQGLKGMCVG EKRKLIIPPA LGYGKEGKGK IPPESTLIFN IDLLEIRNGP RSHESFQEMD LNDDWKLSKD EVKAYLKKEF EKHGAVVNES HHDALVEDIF DKEDEDKDGF ISAREFTYKH DEL
Purity : > 90% by SDS – PAGE.
Form : Liquid in Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.
Concentration : 1 mg/ml (determined by Bradford assay).
Biological activity : Specific activity is > 240 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.
Activity Assay : 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.2. Add 10ul of recombinant FKBP14 protein with 1ug in assay buffer. 3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)5. Record the increase in A405 nm for 30 minutes at 25°C.
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : FKBP14 FK506 binding protein 14, 22 kDa [ Homo sapiens ]
Synonyms : FKBP14; FK506 binding protein 14, 22 kDa; FKBP22; FLJ20731; peptidyl-prolyl cis-trans isomerase FKBP14; FKBP-14; FKBP-22; rotamase; 22 kDa FKBP; PPIase FKBP14; FK506-binding protein 14; 22 kDa FK506-binding protein; EC 5.2.1.8; FK506 binding protein 14 (22 kDa)
Gene ID : 55033
mRNA Refseq : NM_017946
Protein Refseq : NP_060416
UniProt ID : Q9NWM8
Chromosome Location : 7p14.3
Pathway : Diabetes pathways
Function : calcium ion binding; isomerase activity; peptidyl-prolyl cis-trans isomerase activity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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