Recombinant Human PPP3CA, His-tagged

• Cat.No.: PPP3CA-951H
• Product Overview: Recombinant human PPP3CA protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 60.0 kDa (534aa).

Specification

• Cat. No.: PPP3CA-951H
• Description: PPP3CA, also known as Calcineurin A, is a major soluble calmodulin binding protein in the brain, a Ca2+/calmodulin dependent serine/threonine protein phosphatase, with a relatively narrow substrate specificity. It activates the T cells of the immune system and can be blocked by drugs. PPP3CA activates NFATc, a transcription factor by dephosphorylating it. The activated NFATc is then translocated into the nucleus, where it upregulates the expression of interleukin 2.
• Source: E.coli.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 5mM DTT, 1mM EDTA, 20% glycerol.
• Purity: > 85% by SDS - PAGE.
• Concentration: 0.25 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH TGSMSEPKAI DPKLSTTDRV VKAVPFPPSH RLTAKEVFDN DGKPRVDILK AHLMKEGRLE ESVALRIITE GASILRQEKN LLDIDAPVTV CGDIHGQFFD LMKLFEVGGS PANTRYLFLG DYVDRGYFSI ECVLYLWALK ILYPKTLFLL RGNHECRHLT EYFTFKQECK IKYSERVYDA CMDAFDCLPL AALMNQQFLC VHGGLSPEIN TLDDIRKLDR FKEPPAYGPM CDILWSDPLE DFGNEKTQEH FTHNTVRGCS YFYSYPAVCE FLQHNNLLSI LRAHEAQDAG YRMYRKSQTT GFPSLITIFS APNYLDVYNN KAAVLKYENN VMNIRQFNCS PHPYWLPNFM DVFTWSLPFV GEKVTEMLVN VLNICSDDEL GSEEDGFDGA TAAARKEVIR NKIRAIGKMA RVFSVLREES ESVLTLKGLT PTGMLPSGVL SGGKQTLQSA IKGFSPQHKI TSFEEAKGLD RINERMPPRR DAMPSDANLN SINKALTSET NGTDSNGSNS SNIQ.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Alzheimer"s disease; Amyotrophic lateral sclerosis (ALS); Apoptosis; Axon guidance; B cell receptor signaling pathway; Calcium signaling pathway; Long-term potentiation; MAPK signaling pathway; Natural killer cell mediated cytotoxicity; Oocyte meiosis; T cell receptor signaling pathway; VEGF signaling pathway; Wnt signaling pathway; Opioid Signalling

Gene Information

• Gene Name: PPP3CA protein phosphatase 3, catalytic subunit, alpha isozyme [ Homo sapiens ]
• Synonyms: protein phosphatase 3, catalytic subunit, alpha isozyme;CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1; PPP3CA; serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; OTTHUMP00000219551; calcineurin A alpha; CAM-PRP catalytic subunit; calmodulin-dependent calcineurin A subunit alpha isoform; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha); Calmodulin-dependent calcineurin A subunit alpha isoform
• Gene ID: 5530
• mRNA Refseq: NM_000944
• Protein Refseq: NP_000935
• MIM: 114105
• UniProt ID: Q08209
• Chromosome Location: 4q24
• Function: calcium ion binding; calcium-dependent protein serine/threonine phosphatase activity; calmodulin binding; hydrolase activity; protein binding; protein heterodimerization activity; protein serine/threonine phosphatase activity