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Active Recombinant Human PVRL1, His-tagged

Cat.No. : PVRL1-3136H
Product Overview : Recombinant Human PVRL1 (NP_002846.3) extracellular domain (Met 1-Thr 334), fused with a polyhistidine tag at the C-terminus, was produced in Human Cell.
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Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from sterile 50 mM Tris, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : 1. Measured by its binding ability in a functional ELISA. Immobilized PVRL3 at 1 μg/ml (100 μl/well) can bind biotinylated recombinant human PVRL1 / Nectin-1 with a linear range of 6.4-800 ng/ml.
2. Immobilized Nectin 3 Protein, Human, Recombinant (ECD, hFc Tag) at 2 μg/ml (100 μl/well) can bind PVRL1/NECTIN1 Protein, Human, Recombinant (His Tag), the EC50 of PVRL1/NECTIN1 Protein, Human, Recombinant (His Tag)is 150-700 ng/mL.
Molecular Mass : The recombinant human PVRL1 consists of 315 amino acids and predictes a molecular mass of 35.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhPVRL1 is approximately 45-50 kDa due to glycosylation.
Protein Length : Met 1-Thr 334
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 98 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name : PVRL1 poliovirus receptor-related 1 (herpesvirus entry mediator C) [ Homo sapiens ]
Official Symbol : PVRL1
Synonyms : PVRL1; poliovirus receptor-related 1 (herpesvirus entry mediator C); ED4, HVEC; poliovirus receptor-related protein 1; CD111; CLPED1; HIgR; nectin; OFC7; PRR; PRR1; PVRR1; SK 12; nectin 1; poliovirus receptor-like 1; herpesvirus Ig-like receptor; herpes virus entry mediator C; ectodermal dysplasia 4 (Margarita Island type); ED4; HVEC; PVRR; SK-12; nectin-1; MGC16207; MGC142031;
Gene ID : 5818
mRNA Refseq : NM_002855
Protein Refseq : NP_002846
MIM : 600644
UniProt ID : Q15223

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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05/12/2022

    Their technical expertise, product quality, customer support, and supply management collectively contribute to the success and progress of my trials.

    11/29/2019

      Its purity, integrity, and functionality are ensured through stringent quality control measures, which instills confidence in the reliability and accuracy of my research results.

      10/02/2016

        The manufacturer also offers valuable customer support, actively aiding researchers in their experimental journey.

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