Serine (or cysteine) proteinase inhibitor clade B (ovalbumin) member 5, also known as Maspin, is a tumor suppressor protein of serine proteinase inhibitor family. It plays an essential role in embryonic development through critical roles in cell adhesion. Also, it is present in normal breast and prostatic epithelial cells but downregulated in the rspective carcinomas.
Recombinant Maspin (1-375aa) purified from E. coli.
Mouse IgG2b heavy chain and ② light chain.
Anti-human Maspin mAb, clone AT2N6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Maspin protein.
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.
ELISA, WB, Immunofluorescence.
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
The antibody has been tested by ELISA, Western blot analysis and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.
Western Blot Analysis:
Cell lysates of HeLa(20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Maspin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunofluorescence of human HeLa cells stained with PI (Red) and monoclonal anti-Maspin antibody (1:500) with Alexa 488 (Green).