Recombinant Human Vascular Endothelial Growth Factor 165

• Cat.No.: VEGFA-365H
• Product Overview: Recombinant Human Vascular Endothelial Growth Factor (rh-VEGF165) produced in Yeast (Pichia pastoris) is purified via sequential chromatography, 42kDa (165 aa).

Specification

• Cat. No.: VEGFA-365H
• Description: VEGFs are a group of polypeptide growth factors, and they are classified as a member of the platelet-derived growth factor family. They are very potent vascular permeability factors and specific mitogens for vascular endothelial cells, and possess strong angiogenesis in vivo. Among them, VEGF165 is the best potent chemoattractant molecule for monocytes and endothelial cells. It is apparently a homodimer according to reduced and non-reduced SDS-PAGE gels, but preparations of VEGF165 show some heterogeneity, depending on the constitution of different glycosylation patterns. All forms possess similar biological activities.
• Source: Yeast (Pichia pastoris).
• Specific Activity: 1.0 × 105 U/mg.
• Purification: Affinity chromatography using Heparin-Sepharose FF as matrix.
• Sterility: Filtered through a 0.22μm sterile filter.
• Purity: > 90 %, by SDS-PAGE and visualized by silver stain.
• Endotoxin Level: <1.0EU /mg of rh-VEGF165.
• Buffer: 20mM PB, pH7.4.
• Biological Activity: ED50=1~6 ng/ml, determined by the dose dependent proliferation of human umbilical vein cells. Optimal concentration for individual application should be determined by a dose response assay.
• Stability: Liquid rh-VEGF165, although stable at room temperature for no less than 2 weeks, should be stored it below -18℃. For long term storage it is recommended to add a stabilizer PEG4000 (0.1%). Please avoid freeze-thaw cycles.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens ]
• Synonyms: VEGFA; vascular endothelial growth factor A; VPF; VEGF; MVCD1; VEGF-A; MGC70609; vascular permeability factor; vascular endothelial growth factor isoform; VEGF165; vascular endothelial growth factor
• Gene ID: 7422
• mRNA Refseq: NM_001025366
• Protein Refseq: NP_001020537
• MIM: 192240
• UniProt ID: P15692
• Pathway: Bladder cancer; mTOR signaling pathway; Cytokine-cytokine receptor interaction; Focal adhesion; Pancreatic cancer; Pathways in cancer; Renal cell carcinoma; VEGF signaling pathway; Hemostasis; Signaling by VEGF
• Function: cell surface binding; cytokine activity; extracellular matrix binding; fibronectin binding; growth factor activity; heparin binding; platelet-derived growth factor receptor; binding; protein homodimerization activity; vascular endothelial growth factor receptor 1 binding; vascular endothelial growth factor receptor 2 binding