Recombinant Human CRTAM protein, His-tagged

• Cat.No.: CRTAM-3236H
• Product Overview: Recombinant Human CRTAM (Ser18-Gly287) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Class I-restricted T cell-associated molecule (CRTAM), a member of nectin family and the immunoglobulin superfamily, is also known as cytotoxic and regulatory T-cell molecule, which is expressed by activated CD8+ and NK T cells. CRTAM is found in spleen, thymus, small intestine, peripheral blood, and surprisingly, in brain where it is highly expressed by Purkinje cells of the cerebellum. The high affinity of CRTAM/IGSF4 adhesion allows CRTAM to disrupt IGSF4 homotypic interactions. IGSF4 and T cell receptor co-engagement of CRTAM-expressing CD8+ cells induces increased IFN-gamma or IL-22 production. Furthermore, a role in cancer surveillance through NK cell-mediated rejection of IGSF4-expressing tumors has been proposed.
• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Ser18
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 31.9 kDa. The protein migrates as 42-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Ser18-Gly287
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CRTAM
• Official Symbol: CRTAM
• Synonyms: CRTAM; cytotoxic and regulatory T cell molecule; cytotoxic and regulatory T-cell molecule; CD355; class I MHC restricted T cell associated molecule; class-I MHC-restricted T cell associated molecule; class-I MHC-restricted T-cell-associated molecule
• Gene ID: 56253
• mRNA Refseq: NM_019604
• Protein Refseq: NP_062550
• MIM: 612597
• UniProt ID: O95727