| Source : |
Insect Cells |
| Species : |
Human |
| Tag : |
His |
| Form : |
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
1. Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 at 10 μg/ml (100 μl/well) can bind biotinylated human CDK1. The EC50 of biotinylated human CDK1 is 0.02-0.04 μg/ml.2. Measured by its binding ability in a functional ELISA. Immobilized human His-CCNA1 at 10 μg/ml (100 μl/well) can bind biotinylated human CDK2-His. The EC50 of biotinylated human CDK2-His is 0.07-0.15 μg/ml. |
| Molecular Mass : |
The recombinant human CCNA1 consists of 483 amino acids and predicts a molecular mass of 54.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions. |
| Protein Length : |
Met1-Gln465 |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : |
> 96 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
