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Active Recombinant Human GPC5 protein, His-tagged

Cat.No. : GPC5-3332H
Product Overview : Recombinant Human GPC5 protein(NP_004457.1)(Met1-Thr554), fused with His tag, was expressed in Insect Cells.
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Source : Insect Cells
Species : Human
Tag : His
Form : Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized human GPC5 at 5 μg/ml (100 μl/well) can bind human bFGF with a linear range of 0.156-2.5 ng/ml.
Molecular Mass : The secreted recombinant human GPC5 consists of 540 amino acids and predicts a molecular mass of 60.5 kDa as estimated in SDS-PAGE under reducing conditions.
Protein Length : Met1-Thr554
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 92 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : GPC5 glypican 5 [ Homo sapiens ]
Official Symbol : GPC5
Synonyms : GPC5; glypican 5; glypican-5; glypican proteoglycan 5; bA93M14.1;
Gene ID : 2262
mRNA Refseq : NM_004466
Protein Refseq : NP_004457
MIM : 602446
UniProt ID : P78333

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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