Active Recombinant Mouse Il1a protein

• Cat.No.: Il1a-306M
• Product Overview: Recombinant Mouse Il1a protein(NP_034684.2)(Ser115-Ser270) was expressed in E. coli.

Specification

• Source: E. coli
• Species: Mouse
• Tag: N/A
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Immobilized mouse IL1A at 10 μg/mL (100 μl/well) can bind mouse IL1R1, The EC50 of mouse IL1R1 is 0.13 μg/mL.2. Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells.The ED50 for this effect is typically 15-60 ng/mL.
• Molecular Mass: The recombinant mouse IL1A consists of 157 amino acids and migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions as predicted.
• Protein Length: Ser115-Ser270
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il1a interleukin 1 alpha [ Mus musculus ]
• Official Symbol: Il1a
• Synonyms: IL1A; interleukin 1 alpha; interleukin-1 alpha; IL-1 alpha; Il-1a
• Gene ID: 16175
• mRNA Refseq: NM_010554
• Protein Refseq: NP_034684