Recombinant Human PVALB, His-tagged
Cat.No. : | PVALB-2871H |
Product Overview : | Recombinant human PVALB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
- Specification
- Gene Information
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Cat. No. : | PVALB-2871H |
Description : | Parvalbumin alpha, also known PVALB, belongs to a larger group of EF hand proteins. It is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. Parvalbumin is expressed in a specific population of GABAergic interneurones which are thought to play a role in maintaining the balance between excitation and inhibition in the cortex as well as the hippocampus. |
Source : | E.coli. |
Sequence : | MGSSHHHHHH SSGLVPRGSH MGSHMSMTDL LNAEDIKKAV GAFSATDSFD HKKFFQMVGLKKKSADDVKK VFHMLDKDKS GFIEEDELGF ILKGFSPDAR DLSAKETKML MAAGDKDGDG KIGVDEFSTL VAES. |
Purity : | > 95% by SDS – PAGE. |
MW : | 14.6 kDa (134aa) confirmed by MALDI-TOF. |
Form : | Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 2mM DTT, 50mM NaCl, 0.1mM PMSF. |
Concentration : | 1mg/ml (determined by Bradford assay). |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Gene Name : | PVALB parvalbumin [ Homo sapiens ] |
Synonyms : | PVALB; parvalbumin; OTTHUMP00000197806; OTTHUMP00000197807; OTTHUMP00000197808; parvalbumin alpha |
Gene ID : | 5816 |
mRNA Refseq : | NM_002854 |
Protein Refseq : | NP_002845 |
MIM : | 168890 |
UniProt ID : | P20472 |
Chromosome Location : | 22q12-q13.1; 22q13 |
Function : | calcium ion binding |
Products Types
◆ Recombinant Protein | ||
Pvalb-5264M | Recombinant Mouse Pvalb Protein, Myc/DDK-tagged | +Inquiry |
PVALB-4510R | Recombinant Rat PVALB Protein, His (Fc)-Avi-tagged | +Inquiry |
Pvalb-2008R | Recombinant Rat Pvalb Protein, His-tagged | +Inquiry |
PVALB-7306M | Recombinant Mouse PVALB Protein, His (Fc)-Avi-tagged | +Inquiry |
PVALB-2644H | Recombinant Human PVALB Protein, His-tagged | +Inquiry |
◆ Lysates | ||
PVALB-2658HCL | Recombinant Human PVALB 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionPVALB (Parvalbumin) is a calcium-binding protein primarily expressed in fast-spiking inhibitory interneurons of the brain. Its main function is to regulate calcium signaling and buffering within neuronal cells. PVALB acts as a calcium buffer, preventing excessive calcium influx and maintaining proper calcium homeostasis. Additionally, PVALB is involved in modulating synaptic transmission, shaping neuronal network activity, and regulating neuronal excitability, ultimately contributing to the fine-tuning of neuronal circuits.
Emerging evidence suggests that alterations in PVALB expression and function may contribute to the pathogenesis of neurodevelopmental disorders, such as autism spectrum disorders (ASD) and schizophrenia. Reduced PVALB expression has been observed in postmortem brain tissues of individuals with ASD and schizophrenia. Animal models with PVALB deficiencies exhibit behavioral phenotypes resembling certain aspects of these disorders. Dysregulated inhibitory signaling mediated by PVALB interneurons can disrupt the excitatory-inhibitory balance in neural circuits, ultimately leading to cognitive and behavioral impairments associated with neurodevelopmental disorders.
Targeting PVALB has shown potential therapeutic implications in certain neurological disorders. Modulating PVALB expression or activity could potentially restore the disrupted excitatory-inhibitory balance observed in neurodevelopmental disorders. Strategies aimed at enhancing PVALB expression, such as epigenetic modifications or pharmacological interventions, could potentially alleviate cognitive and behavioral deficits in these disorders. However, further research is needed to fully understand the complexity of PVALB-mediated mechanisms in neurological disorders and to develop targeted therapies that specifically modulate PVALB function without interfering with normal neuronal function.
PVALB has been proposed as a potential biomarker for certain neurological diseases. Altered PVALB expression has been observed in several neurodegenerative disorders, including Alzheimer's disease and Parkinson's disease. However, the use of PVALB as a reliable biomarker requires further validation and standardization across different patient populations. Additionally, the development of sensitive and specific assays for measuring PVALB levels in biological samples is necessary to establish its clinical utility as a biomarker. Overall, while PVALB shows promise as a biomarker, more research is needed to establish its diagnostic and prognostic value in neurological diseases.
The expression of PVALB is regulated by various factors, including neuronal activity and transcriptional regulation. Increased neuronal activity, such as enhanced synaptic input, has been shown to upregulate PVALB expression. Transcription factors, such as Lhx6 and Nkx2.1, are involved in the regulation of PVALB gene expression. Additionally, epigenetic modifications, such as DNA methylation and histone acetylation, may also influence PVALB expression levels. However, further research is needed to fully elucidate the precise mechanisms underlying the regulation of PVALB expression in neuronal cells.
The expression of PVALB is tightly regulated during neuronal development and in response to specific physiological and pathological conditions. Studies have identified several transcription factors and signaling pathways involved in the regulation of PVALB expression. For instance, the activity-dependent transcription factor, cAMP response element binding protein (CREB), has been shown to bind to the promoter region of the PVALB gene and regulate its expression in a calcium-dependent manner. Additionally, the Wnt signaling pathway and various growth factors have also been implicated in the modulation of PVALB expression.
PVALB deficiency in the brain leads to various physiological consequences. It has been observed that the loss of PVALB function results in altered neuronal excitability and increased susceptibility to seizures. Impaired calcium buffering due to PVALB deficiency can disrupt synaptic plasticity, leading to deficits in learning and memory processes. Additionally, PVALB deficiency has been associated with imbalanced neuronal network activity and disruptions in the synchronization of neuronal firing patterns. These physiological consequences highlight the crucial role of PVALB in maintaining proper neuronal function and circuit dynamics.
Customer Reviews (3)
Write a reviewWith the assistance of this protein reagent, I am able to more accurately determine protein concentrations, resulting in enhanced precision in my experimental results.
This reagent is safe to use, without any safety concerns.
The manufacturer's proactive suggestions have revolutionized my experimental setup, leading to more accurate results.
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