Recombinant Human Activin A

• Cat.No.: INHBA-2884H
• Product Overview: Human Activin A produced in Insect cells is a 26 kDa disulfide-linked homodimer of two beta A chains, each containing 116 amino acid residues.

Specification

• Cat. No.: INHBA-2884H
• Description: Activin A is a TGF-beta family member that exhibits a wide range of biological activities including regulation of cellular proliferation and differentiation, and promotion of neuronal survival. Elevated levels of Activin A in human colorectal tumors and in post-menopausal woman have been implicated in colorectal and breast cancers, respectively. The biological activities of Activin A can be neutralized by inhibins and by the diffusible TGF-beta antagonist, Follistatin.
• Source: Insect cells.
• Form: Lyophilized.
• Purity: > 95% by SDS-PAGE and HPLC analyses.
• Endotoxin Level: < 0.1 ng per μg of Activin A.
• Biological Activity: The ED50 as determined by its ability to inhibit the proliferation of murine MPC-11 cells is < 2 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg.
• Reconstitution: The human Activin A should be reconstituted in water to a concentration of 0.5 mg/ml. This solution can be stored at 4°C for 1 month or -20°C for future use. It is recommended that further dilutions be made in PBS containing 2 mg/ml albumin and stored at -20°C.
• Stability: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted Human Activin A is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: INHBA inhibin, beta A [ Homo sapiens ]
• Synonyms: INHBA; inhibin, beta A; EDF; FRP; Inhibin, beta-1; OTTHUMP00000207260; FSH-releasing protein; erythroid differentiation factor; follicle-stimulating hormone-releasing protein; inhibin, beta A (activin A, activin AB alpha polypeptide); Activin beta-A chain; Erythroid differentiation protein; FSH-releasing protein
• Gene ID: 3624
• mRNA Refseq: NM_002192
• Protein Refseq: NP_002183
• MIM: 147290
• UniProt ID: P08476
• Chromosome Location: 7p15-p13
• Pathway: Cytokine-cytokine receptor interaction; TGF-beta signaling pathway
• Function: cytokine activity; follistatin binding; growth factor activity; hormone activity; identical protein binding; protein heterodimerization activity; signal transducer activity