Active Recombinant Human PPP1CC, His-tagged

• Cat.No.: PPP1CC-844H
• Product Overview: Recombinant human PPP1CC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Description: The protein encoded by this gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene
• Source: E.coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 50% glycerol, 0.2M NaCl, 2mM DTT
• Bio-activity: Specific activity is > 1200 unit/mg Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.
• Molecular Mass: 39.1 kDa (343aa), confirmed by MALDI-TOF
• Protein length: 1-323aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MADLDKLNID SIIQRLLEVR GSKPGKNVQL QENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYYDLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLPIAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVLGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQVVEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAE KKKPNATRPV TPPRGMITKQ AKK
• Purity: > 85% by SDS - PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Publication:
  Jacob-induced transcriptional inactivation ofCREB promotes Ab-induced synapse loss inAlzheimer’s disease (2023)

Gene Information

• Gene Name: PPP1CC protein phosphatase 1, catalytic subunit, gamma isozyme [ Homo sapiens ]
• Official Symbol: PPP1CC
• Synonyms: PPP1CC; protein phosphatase 1, catalytic subunit, gamma isozyme; PPP1G; PP1gamma; erine/threonine-protein phosphatase PP1-gamma catalytic subunit; PP-1G; serine/threonine phosphatase 1 gamma; protein phosphatase 1C catalytic subunit; protein phosphatase 1, catalytic subunit, gamma isoform; EC 3.1.3.16
• Gene ID: 5501
• mRNA Refseq: NM_002710
• Protein Refseq: NP_002701
• MIM: 176914
• UniProt ID: P36873
• Chromosome Location: 12q24.1-q24.2
• Pathway: Focal adhesion; Insulin signaling pathway; Long-term potentiation; Oocyte meiosis; Regulation of actin cytoskeleton; Vascular smooth muscle contraction
• Function: hydrolase activity; metal ion binding; protein binding; protein kinase binding; protein serine/threonine phosphatase activity