Active Recombinant Human HDAC3, FLAG-tagged

• Cat.No.: HDAC3-854H
• Product Overview: Recombinant HumanHDAC3 is expressed in Baculovirus with a FLAG tag at N-terminus.

Specification

• Cat. No.: HDAC3-854H
• Description: The nucleosomal histones can be modified through reversible acetylation by histone acetyltransferases (HATs) and deacetylases (HDACs). Acetylation and deacetylation of histone proteins alters chromosome structure and affects transcription factor access to DNA. HDAC3 has an open reading frame of 428 amino acids. HDAC3 shares 53% amino acid identity with HDAC1 and 52% with HDAC2.
• Source: Baculovirus.
• Formulation: 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 0.2 mM EDTA and 1mM DTT.
• Application: Recombinant HDAC3 can be used 1) for in vitro function studies including transcription in the presence of co-factors such as N-CoR; 2) for protein-protein interaction assay; and 3) for cell growth assay.
• Activity: 1 unit equals 1 nanogram of purified protein.
• Publication:
  Circumvention of Gefitinib Resistance by Repurposing Flunarizine via Histone Deacetylase Inhibition (2023)

Gene Information

• Gene Name: HDAC3 histone deacetylase 3 [ Homo sapiens ]
• Synonyms:
  HDAC3; ; histone deacetylase 3; RPD3; HD3; RPD3-2; EC 3.5.1.98; SMAP45
• Gene ID: 8841
• mRNA Refseq: NM_003883
• Protein Refseq: NP_003874
• MIM: 605166
• UniProt ID: O15379
• Chromosome Location: 5q31
• Pathway: Signalling by NGF
• Function: DNA binding; histone deacetylase activity; hydrolase activity; transcription factor binding