Recombinant Human POLK Protein, His-tagged

• Cat.No.: POLK-763H
• Product Overview: This Human POLK Protein was over-expressed as a recombinant protein in E. coli with a plasmid carrying a C-terminal histidine-tagged human DNA polymerase (1-560 aa), and highly purified by several steps of chromatography.

Specification

• Description: This gene encodes a member of the DNA polymerase type-Y family of proteins. The encoded protein is a specialized DNA polymerase that catalyzes translesion DNA synthesis, which allows DNA replication in the presence of DNA lesions. Human cell lines lacking a functional copy of this gene exhibit impaired genome integrity and enhanced susceptibility to oxidative damage.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: 65 kDa
• Purity: > 90% by SDS-PAGE (CBB staining)
• Applications: 1) Functional studies on translesion DNA synthesis by DNA polymerase kappa
  2) Antigen for western blotting and ELISA by anti-Pol kappa antibody
• Storage: Sent at 4 centigrade or at -20 centigrade. Upon arrival, centrifuge briefly and store at -20 centigrade or at -80 centigrade for longer storage.
• Concentration: 3.2 mg/mL as measured by BCA method
• Storage Buffer: 0.2 M NaCl, 10 mM sodium phosphate buffer (pH 7.0), 50% glycerol

Gene Information

• Gene Name: POLK
• Official Symbol: POLK DNA polymerase kappa [ Homo sapiens (human) ]
• Synonyms: POLK; DNA polymerase kappa; DINP; POLQ; DINB1; DNA polymerase kappa; polymerase (DNA directed) kappa; EC 2.7.7.7
• Gene ID: 51426
• mRNA Refseq: NM_016218
• Protein Refseq: NP_057302
• MIM: 605650
• UniProt ID: Q9UBT6