Recombinant Human Maternal Embryonic Leucine Zipper Kinase

• Cat.No.: MELK-730H
• Product Overview: RecombinantHuman MELK, N-terminal fragment, amino acids M1-A342, untagged, was expressed inSf9insect cells. MW = 39,852 Da.

Specification

• Cat. No.: MELK-730H
• Description: Maternal embryonic leucine zipper kinase is an enzyme that in humans is encoded by the MELK gene.MELK has been shown to interact with CDC25B.
• Source: Baculovirus infected Sf9 cells.
• Purification: GST-Affinity Chromatography followed by 3C mediated removal of the GST tag.
• Product Identity: MELK was confirmed as MELK by mass spectroscopy LC-ESI-MS/MS.
• Storage Buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol.
• Concentration: 0.191 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 6 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: MELK maternal embryonic leucine zipper kinase [ Homo sapiens ]
• Synonyms: MELK; maternal embryonic leucine zipper kinase; HPK38; KIAA0175; Hmelk; pEg3 kinase; protein kinase PK38; EC 2.7.11.1; Protein kinase PK38
• Gene ID: 9833
• mRNA Refseq: NM_014791
• Protein Refseq: NP_055606
• MIM: 607025
• UniProt ID: Q14680
• Chromosome Location: 9p13.2
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity