Recombinant Human VHL fused with His tag was expressed in Insect cells.
The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70 centigrade before use. Avoid repeated freeze thaw cycles.
MPRRAENWDE AEVGAEEAGV EEYGPEEDGG EESGAEESGP EESGPEELGA EEEMEAGRPRPVLRSVNSRE PSQVIFCNRS PRVVLPVWLN FDGEPQPYPT LPPGTGRRIH SYRGHLWLFRDAGTHDGLLV NQTELFVPSL NVDGQPIFAN ITLPVYTLKE RCLQVVRSLV KPENYRRLDIVRSLYEDLED HPNVQKDLER LTQERIAHQR MGD
The purified VHL is greater than 90% homogeneous based on SDS-PAGE analysis.
1 unit equals 1 nanogram of purified protein. 30-100 units (ng) are required for an in vitro transcription assay and protein-protein interaction assay.
Purified VHL protein has been used for in vitro transcriptional activation and protein-protein interaction assays.