"VHL" Related Products

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Recombinant Human VHL protein, His-tagged

Cat.No.: VHL-2517H
Product Overview: Recombinant Human VHL fused with His tag was expressed in Insect cells.
Source: Insect cells
Species: Human
Tag: His
Form: The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70 centigrade before use. Avoid repeated freeze thaw cycles.
Molecular Mass: 26.0 kDa
AA Sequence: MPRRAENWDE AEVGAEEAGV EEYGPEEDGG EESGAEESGP EESGPEELGA EEEMEAGRPRPVLRSVNSRE PSQVIFCNRS PRVVLPVWLN FDGEPQPYPT LPPGTGRRIH SYRGHLWLFRDAGTHDGLLV NQTELFVPSL NVDGQPIFAN ITLPVYTLKE RCLQVVRSLV KPENYRRLDIVRSLYEDLED HPNVQKDLER LTQERIAHQR MGD
Purity: The purified VHL is greater than 90% homogeneous based on SDS-PAGE analysis.
Unit Definition: 1 unit equals 1 nanogram of purified protein. 30-100 units (ng) are required for an in vitro transcription assay and protein-protein interaction assay.
Applications: Purified VHL protein has been used for in vitro transcriptional activation and protein-protein interaction assays.
Gene Name: VHL von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase [ Homo sapiens ]
Official Symbol: VHL
Synonyms: VHL; von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase; von Hippel Lindau syndrome , von Hippel Lindau tumor suppressor; von Hippel-Lindau disease tumor suppressor; VHL1; protein G7; elongin binding protein; RCA1; pVHL; HRCA1;
Gene ID: 7428
mRNA Refseq: NM_000551
Protein Refseq: NP_000542
MIM: 608537
UniProt ID: P40337
Chromosome Location: 3p25.3
Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; ECV complex, organism-specific biosystem; HIF-2-alpha transcription factor network, organism-specific biosystem;
Function: enzyme binding; protein binding; transcription factor binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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