Recombinant Human TIMP Metallopeptidase Inhibitor 3

• Cat.No.: TIMP3-2528H

Specification

• Cat. No.: TIMP3-2528H
• Description: Metalloproteinase inhibitor 3 is an enzyme that in humans is encoded by the TIMP3 gene. This gene belongs to the tissue inhibitor of metalloproteinases gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby"s fundus dystrophy.
• Source: Mammalian cells
• Analysis Note: The biological activity is measured by its ability to inhibit human MMP-2 hydrolysis of a peptide substrate.
• Biochem/physiol Actions: The TIMPs are endogenous inhibitors of the matrix metalloproteinases (MMPs). TIMP-3 inhibits ADAM-17 (TACE) at nanomolar concentrations and also inhibits other metalloproteinases (sheddases) that mediate the shedding of soluble receptors and other proteins from the surface of cells.
• Physical Form: Lyophilized from a 0.2 μm filtered solution in 25 mM Tris and 0.15 M sodium chloride, pH 7.5.
• Assay: >95% (SDS-PAGE).
• Form: buffered aqueous solution.
• Mol Wt: apparent mol wt ~30 kDa.
• Storage Temp: −20°C.

Gene Information

• Gene Name: TIMP3 TIMP metallopeptidase inhibitor 3 [ Homo sapiens ]
• Synonyms: TIMP metallopeptidase inhibitor 3; SFD; K222; K222TA2; HSMRK222; TIMP3; metalloproteinase inhibitor 3; TIMP-3; MIG-5 protein; protein MIG-5; tissue inhibitor of metalloproteinases 3; tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, pseudoinflammatory); Protein MIG-5; Tissue inhibitor of metalloproteinases 3
• Gene ID: 7078
• mRNA Refseq: NM_000362
• Protein Refseq: NP_000353
• MIM: 188826
• UniProt ID: P35625
• Chromosome Location: 22q12.1-q13.2; 22q12.3
• Function: enzyme inhibitor activity; metal ion binding; metalloendopeptidase inhibitor activity; protein binding