Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Activity Kits > DNA Methyltransferase Activity/Inhibition Assay Kit (Fluorometric)

DNA Methyltransferase Activity/Inhibition Assay Kit (Fluorometric)

"DNMT" Related Products.
Product Overview:
DNA Methyltransferase Activity/Inhibition Assay Kit (Fluorometric) is use for measuring Dnmt activity/inhibition.
Epigenetic inactivation of genes plays a critical role in many important human diseases, especially in cancer. A core mechanism for epigenetic inactivation of the genes is methylation of CpG islands in genome DNA. Methylation of CpG islands involves the course in which DNA methyltransferases (Dnmts) transfer a methyl group from S-adenosyl-L-methionine to the fifth carbon position of the cytosines. Four active Dnmts have been identified in mammals. They are named DNMT1, DNMT2, DNMT3A, and DNMT3B. Inhibition of Dnmts maylead to demethylation and expression of the silenced genes, thus Dnmt inhibitors are currently being developed as potential anticancer agents.
For measuring Dnmt activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.
For research use only (RUO)
Store MD3, MD4, MD6, and MD7 at –20°C away from light. Store all other components at 4°C away from light.The kit is stable for up to 6 months from date of shipment when stored properly.
Kit Components:
MT1 (10X wash buffer) 22 mlMT2 (Dnmt assay buffer) 3 mlMT3 (Adomet, 8 mM)* 70 µlMD4 (Dnmt positive control)* 10 µlMT5 (capture antibody)* 8 µlMT6 (detecting antibody 200 µg/ml)* 20 µlMD7 (fluoro developer)* 24 µlMD8 (fluoro enhancer)* 24 µlMD9 (fluoro dilution) 8 ml8 well substrate-coated strip (with frame) 12* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.
Features & Benefits:
Extremely fast procedure, which can be completed within 3 hours.Innovative fluorometric assay with high sensitivity for detecting Dnmt activity generated from as low as 2 ng of enzyme.Strip microplate format makes the assay flexible: manual or high throughput analysis.Simple, reliable, and consistent assay conditions.

Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

facebook twitter linkedin google plus google plus

Join 10 other subscribers

Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2017 Creative Biomart. All Rights Reserved.