Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email:
Home > Products > Assay Kits > Activity Kits > DPPIV/CD26 Assay Kit

DPPIV/CD26 Assay Kit

"DPP4" Related Products.
96 wells
Because DPPIV/CD26 protein levels may not accurately reflect the levels of active DPPIV enzyme, it is useful to measure DPPIV activity rather than performing DPPIV immunoassay. The DPPIV/CD26 Assay kit for biological samples is a complete assay system designed to measure DPPIV activity in biological fluids such as plasma, serum, urine, and saliva. Uses for the kit include correlation of DPPIV activity with disease states or determination of the efficacy of DPPIV inhibitors administered in vivo. The kit can easily be used for other biological fluids such as tissue, live cells and cell extracts, and exudates. Enough reagents are provided to perform at least 96 assays. The kit contains both a chromogenic substrate (H-Gly-Pro-pNA) and a fluorogenic substrate (H-Gly-Pro-AMC). Cleavage of the p-nitroaniline (pNA) from the chromogenic substrate increases absorbance at 405 nm. The fluorimetric assay is based on the cleavage of 7-amino-4-methylcoumarin (AMC) moiety from the C-terminus of the peptide substrate, which increases its fluorescence intensity at 460 nm.
Colorimetric detection, Fluorescence microscopy. Correlation of DPPIV activity with disease states, or determination of the efficacy of DPPIV inhibitors administered in vivo.
Kit Components:
DPPIV enzyme (Human, recombinant) (5 mU; One U=1μmole/min at 37°C, 100µM H-Gly-Pro-pNA. Purity >95% (SDS-Page)), Storage: -70°C; avoid freeze thaw cyclespNA substrate (H-Gly-Pro-pNA) (55µl; 100mM in DMSO)Storage: -70°CpNA calibration standard (p-nitroaniline) (600μL; 80µM in 50mM Glycine, pH 8.7, 1mM EDTA)Storage: -70°CAMC substrate (H-Gly-Pro-AMC) (45µl; 50mM in DMSO), Storage: -70°CAMC calibration standard (7-amino-4-methylcoumarin) (600μL; 50µM in 50mM Glycine, pH 8.7, 1mM EDTA), Storage: -70°CInhibitor P32/98 (Prod. No. BML-PI142-9090) (20µl; 1mM in DMSO), Storage: -70°CAssay buffer (20ml; 50mM Glycine, pH 8.7, 1mM EDTA; liquid in screw-cap plastic bottle), Storage: -20 or -70°C½-volume clear microplate, Storage: Room temperature½-volume NBS white microplate, Storage: Room temperature

Online Inquiry    
Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
Please input "biomart" as verification code.
Please review Creative Biomart's privacy policy for more information.
Price does not include shipping and packaging costs.Request quote for final price.

If you have any other questions, please call

online inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127

facebook twitter linkedin google plus google plus

Join 10 other subscribers

Easy access to products and services you need from our library via powerful searching tools
Copyright © 2007 - 2017 Creative Biomart. All Rights Reserved.