"Enterokinase" Related Products

Enteropeptidase/Enterokinase Activity Fluorometric Assay Kit

Cat.No.: Kit-1106
Description: Enteropeptidase (Enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence ‘DDDDK’ and cleaves after the lysine residue. High specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion protein tags containing the above recognition motif. In Enteropeptidase Activity Assay Kit, we have utilized a peptide substrate containing the Enteropeptidase recognition sequence along with a fluorescent label ‘AFC’. Enteropeptidase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. This assay kit is simple and rapid and can detect Enteropeptidase activity as low as 1 mU.
Applications: Measurement of Enteropeptidase activity in biological samples or purified Enteropeptidase activity.
Removing tag from recombinant proteins having recognition motif.
Storage: -20°C
Shipping: Gel Pack
Size: 100 assays
Kit Components: Enteropeptidase Assay Buffer; Enteropeptidase Substrate (10 mM, in DMSO); Human Enteropeptidase (Positive Control); AFC Standard (1 mM)
Detection method: Fluorescence(Ex/Em = 380/500 nm)
Features & Benefits: Simple & convenient procedure.
Can detect enteropeptidase activity as low as 1 mU.
Kit contains all necessary reagents for measuring enteropeptidase activity.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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