Glutamic Oxaloacetic Transaminase Activity Assay Kit

• Cat.No.: Kit-0381
• Product Overview: Glutamic Oxaloacetic Transaminase Activity Assay Kit an amino group is transferred of from aspartate to alpha-ketoglutarate. The products of this reversible transamination reaction are oxaloacetate and glutamate. The glutamate is detected in a reaction that concomitantly converts a nearly colorless probe to color (λmax = 450 nm). The kit provides a rapid, simple, sensitive and reliable test suitable as a high throughput activity assay of GOT with a detection limit of 10 mU per well.

Specification

• Size: 100 x 1 assay
• Applications: Functional Studies
• Storage: Store the kit at -20°C protected from light. Allow the Assay Buffer to warm to room temperature before use. Briefly centrifuge vials before opening. Read the entire protocol before performing the assay.GOT ENZYME MIX: Reconstitute with 220 μL dH2O. Aliquot and store at -20°C. Use within two months.GOT DEVELOPER: Reconstitute with 820 μL dH2O. Aliquot and store at -20°C. Use within two monthsGOT SUBSTRATE: Reconstitute with 1.1 ml assay buffer. Store at -20°C. Use within two months.5GOT POSITIVE CONTROL: Reconstitute with 100 μL dH2O. Aliquotand store at -20°C. Use within two months. In the assay (optional),add 5 μL positive control and adjust the volume to 50 μL/well with Assay Buffer.