"BDNF" Related Products

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GMP Recombinant Human BDNF Protein

Cat.No.: BDNF-113HG
Product Overview: Recombinant Human BDNF, His129-Arg247, was produced in Sf 21 (baculovirus)-derived in an animal component free process under cGMP guidelines.
Source: Insect cell
Species: Human
Form: Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and NaCl.
Bio-activity: Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.2-2 ng/mL.
The specific activity of Recombinant Human/Mouse/Rat/Canine/Equine BDNF is approximately 1.3 x 10^3 units/μg, which is calibrated against recombinant human BDNF WHO Standard (NIBSC code: 96/534).
Molecular Mass: Predicted Molecular Mass: 13.5 kDa
SDS-PAGE: 13-14 kDa, reducing conditions
N-terminal Sequence Analysis: His129-Ser-Asp-Pro-Ala-Arg-Arg-Gly-Glu-Leu
Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
Purity: >97%, by SDS-PAGE with silver staining, under reducing conditions.
Storage: Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100 μg/mL in PBS.
Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
Gene Name: BDNF brain-derived neurotrophic factor [ Homo sapiens ]
Official Symbol: BDNF
Synonyms: BDNF; brain-derived neurotrophic factor; neurotrophin; abrineurin; MGC34632;
Gene ID: 627
mRNA Refseq: NM_001143805
Protein Refseq: NP_001137277
MIM: 113505
UniProt ID: P23560

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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