Active GMP Recombinant Human FGF2 Protein

• Cat.No.: FGF2-108HG
• Product Overview: Recombinant Human FGF2, Pro143-Ser288, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.

Specification

• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
• Bio-activity: Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL.
  The specific activity of Recombinant Human FGF basic GMP is approximately 800 IU/μg, which is calibrated against human FGF basic WHO International Standard (NIBSC code: 90/712).
• Molecular Mass: Predicted Molecular Mass: 16.5 kDa
  SDS-PAGE: 17 kDa, reducing conditions
• N-terminal Sequence Analysis: Ala-Pro143-Ala-Leu-Pro-Glu-Asp-Gly-Gly-Ser
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE with silver staining.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: FGF2 fibroblast growth factor 2 (basic) [ Homo sapiens ]
• Official Symbol: FGF2
• Synonyms: FGF2; fibroblast growth factor 2 (basic); FGFB; fibroblast growth factor 2; prostatropin; BFGF; FGF-2; HBGF-2
• Gene ID: 2247
• mRNA Refseq: NM_002006
• Protein Refseq: NP_001997
• MIM: 134920
• UniProt ID: P09038