Active GMP Recombinant Human GDF11 Protein

• Cat.No.: GDF11-137HG
• Product Overview: Recombinant Human GDF11, Asn299-Ser407, was produced in E. coli using non-animal reagents in an animal-free facility under cGMP guidelines.

Specification

• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Bio-activity: Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340.
• Molecular Mass: Predicted Molecular Mass: 12.6 (monomer) kDa
  SDS-PAGE: 12 kDa, reducing conditions
• N-terminal Sequence Analysis: Met-Asn299-Leu-Gly-Leu-Asp-(Cys)-Asp-Glu-His
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in 4 mM HCl.
• Quality Statement: Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: GDF11 growth differentiation factor 11 [ Homo sapiens ]
• Official Symbol: GDF11
• Synonyms: GDF11; growth differentiation factor 11; growth/differentiation factor 11; BMP 11; GDF-11; bone morphogenetic protein 11; BMP11; BMP-11
• Gene ID: 10220
• mRNA Refseq: NM_005811
• Protein Refseq: NP_005802
• MIM: 603936
• UniProt ID: O95390