|Product Overview:||Recombinant Human GDNF, Arg109-Ile211, was produced in NS0 cell under cGMP guidelines.|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity:||Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells.
The specific activity of recombinant human GDNF is approximately 3.1 x 10^3 units/μg, which is calibrated against recombinant human GDNF Reference Standard (NIBSC code: 09/266).
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera at 1 µg/mL can bind Recombinant Human GDNF with an apparent Kd <1 nM.
|Molecular Mass:||Predicted Molecular Mass: 11.6 (monomer) kDa|
|N-terminal Sequence Analysis:||Arg109-Gly-Gln-Arg-Gly-Lys-Asn-Arg-Gly-(Cys)|
|Endotoxin:||<1.0 EU per 1 μg of the protein by the LAL method.|
|Purity:||>97%, by SDS-PAGE with silver staining, under reducing conditions.|
|Storage:||Avoid repeated freeze-thaw cycles.
12 months, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 100 μg/mL in PBS.|
|Quality Statement:||Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.|
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||GDNF glial cell derived neurotrophic factor [ Homo sapiens ]|
|Synonyms:||GDNF; ATF1; ATF2; glial cell line derived neurotrophic factor; glial derived neurotrophic factor; HFB1 GDNF; ATF; astrocyte-derived trophic factor; HSCR3; HFB1-GDNF;|
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