Active GMP Recombinant Human HGF Protein
Cat.No. : | HGF-143HG |
Product Overview : | Recombinant Human HGF, Gln32-Ser728, was produced in NS0 cell under cGMP guidelines. |
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Source : | Mammalian cells |
Species : | Human |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS. |
Bio-activity : | Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The specific activity of recombinant human HGF is approximately 1.5 x 10^3 IU/μg, which is calibrated against recombinant human HGF WHO International Standard (NIBSC code: 96/564). |
Molecular Mass : | Predicted Molecular Mass: alpha chain: 53.7; beta chain: 26 kDa SDS-PAGE: 65-75 kDa, non-reducing conditions 60-65 kDa, and 30-40 kDa, reducing conditions |
N-terminal Sequence Analysis : | Amino acid sequencing of the alpha chain was blocked, suggesting it is consistent with Gln32 Gln32-Arg-Lys-Arg-Arg-Asn-Thr-Ile-His-Glu (alpha -chain) Val495-Val-Asn-Gly-Ile-Pro-Thr-Arg-Thr-Asn (beta -chain) |
Endotoxin : | <0.10 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE with silver staining, under reducing conditions. |
Storage : | Avoid repeated freeze-thaw cycles. 12 months, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 100 μg/mL in PBS. |
Quality Statement : | Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing. |
Shipping : | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Gene Name : | HGF hepatocyte growth factor (hepapoietin A; scatter factor) [ Homo sapiens ] |
Official Symbol : | HGF |
Synonyms : | HGF; hepatocyte growth factor; F TCF; fibroblast derived tumor cytotoxic factor; hepatopoietin A; HGFB; HPTA; scatter factor; SF; hepatopoeitin-A; hepatopoietin-A; F-TCF; DFNB39; |
Gene ID : | 3082 |
mRNA Refseq : | NM_000601 |
Protein Refseq : | NP_000592 |
MIM : | 142409 |
UniProt ID : | P14210 |
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HGF-18H | Active Recombinant Human HGF Protein, Animal Free | +Inquiry |
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◆ Native Protein | ||
HGF-38P | Native Porcine HGF | +Inquiry |
HGF-29231TH | Native Human HGF | +Inquiry |
◆ Lysates | ||
HGF-1233RCL | Recombinant Rat HGF cell lysate | +Inquiry |
HGF-1210MCL | Recombinant Mouse HGF cell lysate | +Inquiry |
HGF-1077CCL | Recombinant Cynomolgus HGF cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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